Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Tylenol and glutathione, actually explainedhere's the bullet points: * Lots of concern raised on social media re: glutathione depletion and acetaminophen use * Glutathione is used in the metabolism of acetaminophen and Oxidative stress during acetaminophen hepatotoxicity: Sources, pathophysiological role and therapeutic potential ScienceDirect Paracetamol toxicity Biochemical Basis Lecture Haggling with HAGMA: A Common Medication with an Uncommon Differential Renal Fellow Network Can Tylenol (acetaminophen) deplete glutathione levels in the body?